Live-cell imaging of small nucleolar RNA tagged with the broccoli aptamer in yeast
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چکیده
منابع مشابه
Live Cell Imaging in Fission Yeast.
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Small non-coding RNAs (sRNAs) are an emerging class of post-transcriptional regulators of bacterial gene expression. To study sRNAs and their potential protein interaction partners, it is desirable to purify sRNAs from cells in their native form. Here, we used RNA-based affinity chromatography to purify sRNAs following their expression as aptamer-tagged variants in vivo. To this end, we develop...
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H/ACA small nucleolar RNPs (snoRNPs) that guide pseudouridylation reactions are comprised of one small nucleolar RNA (snoRNA) and four common proteins (Cbf5, Gar1, Nhp2 and Nop10). Unlike other H/ACA snoRNPs, snR30 is essential for the early processing reactions that lead to the production of 18S ribosomal RNA in the yeast Saccharomyces cerevisiae. To determine whether snR30 RNP contains specif...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2018
ISSN: 1567-1364
DOI: 10.1093/femsyr/foy093